Mentor Areas
- Radiation Biology
- Stem cell Biology
- Normal tissue response to radiation
- Molecular and tumor biology
- Pancreatic tumor microenvironment
Description:
Radiotherapy (RT) is a common treatment for abdominal cancers like pancreatic tumors. Despite advancements in RT delivery, its effectiveness is hindered by the proximity of the radiosensitive intestine.
To understand the intestinal epithelial regeneration and the molecular mechanisms of RT-induced toxicities, we developed a clinically relevant mouse model of focal RT, by implanting a radiopaque marker on the jejunum. We have previously shown that interleukin-6 is necessary for epithelial regeneration post-focal irradiation. Intriguingly, we observed increased crypt length and stem cell proliferation at adjacent non-irradiated sites (wound edge). A dose escalation (up to 32 Gy) resulted in 100% survival for focally irradiated mice, while whole abdomen IR led to mortality within a week post-irradiation. This prompted us to explore deeper into understanding how stem cells from adjacent non-irradiated regions contribute to the regeneration of the radiation-damaged site. Through the use of single-cell RNA sequencing, spatial transcriptomic, and genetically engineered mouse models, our goal is to unravel the mechanisms underlying intestinal regeneration. This research has the potential to enhance therapeutic outcomes and improve the quality of life for patients.
Preferred Qualifications
Basic lab experience
Preferred experience in immunofluorescent staining
Preferred experience in mouse handling
Project Website
Learn more about the researcher and/or the project here. https://www.verginadislab.org/?p=16
Details:
Preferred Student Year
First-year, Second-Year
Academic Term
Fall, Spring, Summer
I prefer to have students start during the above term(s).Volunteer
No
Yes indicates that faculty are open to volunteers.Paid
Yes
Yes indicates that faculty are open to paying students they engage in their research, regardless of their work-study eligibility.Work Study
Yes
Yes indicates that faculty are open to hiring work-study-eligible students.